11444 Lrtm1 Status: Cancelled
| Gene | Lrtm1 |
| Chromosome | 14 |
| Deletion Start | 29,018,552 |
| Deletion End | 29,027,755 |
| Deletion Size | 9,204 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,019,159 | 29,019,181 | TGGATGCTCTATGTCTCTTGAAC |
| TUP | 29,019,185 | 29,019,210 | ATCAGAGGCAGCAGCTTTCCCTTGGA |
| TUR | 29,019,264 | 29,019,284 | CTTGATCTCCCTGCTTTGGAC |
| TDF | 29,027,530 | 29,027,550 | GAGGCCAAACCCAAACCAAAG |
| TDP | 29,027,552 | 29,027,574 | CAACCAACCTGCGCCATGCTGTG |
| TDR | 29,027,595 | 29,027,619 | GAGACATACGATCCCACATACTACC |
PCR Primers
| Primer | Sequence |
| SU | GGACCGGACGCAATCAAAAG |
| SD | CAGTTGCAAGATATGAGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 284 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
