11447 Calml3 Status: Cancelled
| Gene | Calml3 |
| Chromosome | 13 |
| Deletion Start | 3,804,200 |
| Deletion End | 3,803,732 |
| Deletion Size | 469 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,804,110 | 3,804,131 | GATGGCAGTATCACCACCCAAG |
| TUP | 3,804,086 | 3,804,108 | ATTGGGCACTGTCATGCGGTCCC |
| TUR | 3,804,014 | 3,804,033 | CGGTGCCGTTTCCATCTTTG |
| TDF | 3,803,840 | 3,803,860 | AGAAGCTGAGCGATGAGGAAG |
| TDP | 3,803,817 | 3,803,839 | TGGACGAAATGATACAGGCGGCA |
| TDR | 3,803,762 | 3,803,781 | ACACCAGCATGTGGACAAAC |
PCR Primers
| Primer | Sequence |
| SU | ATCCTACCAGACCAACGATG |
| SD | AAGAACTGGTGTGAAGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 309 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
