11460 Rasl12 Status: Cancelled
| Gene | Rasl12 |
| Chromosome | 9 |
| Deletion Start | 65,398,568 |
| Deletion End | 65,411,164 |
| Deletion Size | 12,597 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,398,776 | 65,398,796 | TTCTCTGTTGCAGGCTATCAC |
| TUP | 65,398,797 | 65,398,818 | TCTCGCCTTGCTTCTCCATCGC |
| TUR | 65,398,828 | 65,398,846 | GGCAGGCAAGCAGCTAAAG |
| TDF | 65,410,737 | 65,410,758 | TGCAATGACATCCTCCTGACTG |
| TDP | 65,410,765 | 65,410,788 | CCTGCTTCCTCTCCAGGCAAGTCA |
| TDR | 65,410,807 | 65,410,827 | ACCCGAATCTGCCTGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGGCAAGGAGTGCAAC |
| SD | TGAGGCAGTCAGGAGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 286 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
