11461 Rgag1 Status: Cancelled
| Gene | Rgag1 |
| Chromosome | X |
| Deletion Start | 143,099,579 |
| Deletion End | 143,104,315 |
| Deletion Size | 4,737 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,100,477 | 143,100,497 | CCCAGCCAACAAATCCTCCAG |
| TUP | 143,100,500 | 143,100,526 | TCTGAAGGGATACCCACTGTGCTCATG |
| TUR | 143,100,569 | 143,100,586 | TGCTCCAGAGCCAGAAAC |
| TDF | 143,103,463 | 143,103,483 | GCTGGCTGAAGAGAAGGATTC |
| TDP | 143,103,486 | 143,103,511 | CAGGCAACTCAAACCAAGTTGTGCCA |
| TDR | 143,103,517 | 143,103,539 | CCTCCTCATTGTTCCGCTTACAG |
PCR Primers
| Primer | Sequence |
| SU | TCTTCAAACTGATAGACCCAC |
| SD | ACACTCATATAGTCCACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 317 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
