11462   Ccl21c     Status: Cancelled

GeneCcl21c
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1891386
NCBI Gene ID65956
BAC ID115O1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF41,903,75541,903,774CCAAGGAGGGAGAGGCTTTG
TUP41,903,78941,903,818CCATAGAGACACCTTATAAGCCGCAGCTGG
TUR41,903,85641,903,879CTGTACTTAAGGCAGCAGTCCTGT
TDF41,904,04041,904,060CCCTCCAACCCTCAGGTTCTT
TDP41,904,06241,904,084CCCCGGAAGCACTCTAAGCCTGA
TDR41,904,10441,904,123AGGTTCTGCACCCAGCCTTC

PCR Primers

PrimerSequence
SUAGCTGAACAGCTAGGAGCTG
SDTCTGACTCTCTAGGTCTACC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR125 bp++-
TDFTDR84 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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