11463 Nkiras1 Status: Cancelled
| Gene | Nkiras1 |
| Chromosome | 14 |
| Deletion Start | 18,276,716 |
| Deletion End | 18,280,230 |
| Deletion Size | 3,534 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,277,609 | 18,277,629 | TCTGTAGGCCAGGCTGATTTG |
| TUP | 18,277,631 | 18,277,653 | AGCTCCGAGACCACCTGCCTCTG |
| TUR | 18,277,660 | 18,277,680 | ACGCCTTCCTTTCCAGCACTC |
| TDF | 18,279,983 | 18,280,004 | TCTGAGCAGAGACAGGTGGATG |
| TDP | 18,280,008 | 18,280,030 | ACGTGGCACAACAGTGGGCAAGA |
| TDR | 18,280,036 | 18,280,055 | CCTCCCACAGCTTGACTTTC |
PCR Primers
| Primer | Sequence |
| SU | GCATGGAACTGAGACCGATC |
| SD | TACAAGTTCTGCAAGGCGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 419 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 506 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
