11465 Accn2 Status: ES cell colonies screened / QC no positives
| Gene | Accn2 |
| Chromosome | 15 |
| Deletion Start | 99,671,803 |
| Deletion End | 99,699,151 |
| Deletion Size | 2,436 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGAGCTTGAAGCGAGACGATAC |
| TUP | | | AAGTCTTAAGTTTCCCATAACCTGCCTGGA |
| TUR | | | TGCGGGCAGAGTTTCCTTG |
| TDF | | | TCAAACCTGCGCCAAAGAAG |
| TDP | | | TCTTGCAATACCTCGCGCGCTTTG |
| TDR | | | AGGTGGCTGACGAGCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GCCAACTGCTTCACACTTTC |
| SD | ATCAGGGAGGCTCCTTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
