11468 Fpr-rs6 Status: Cancelled
| Gene | Fpr-rs6 |
| Chromosome | 17 |
| Deletion Start | 20,183,094 |
| Deletion End | 20,182,052 |
| Deletion Size | 1,043 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,182,692 | 20,182,714 | GCATCCAGTATGGGCTCAGAATC |
| TUP | 20,182,662 | 20,182,689 | CGAACTGTGAATGTGGCAACGAAAGTGA |
| TUR | 20,182,636 | 20,182,660 | CAGAACAAGTATCCAAGCTCCAAAG |
| TDF | 20,182,438 | 20,182,465 | CATACCAATGATCTTCATTGTCATCTGT |
| TDP | 20,182,411 | 20,182,436 | ATGGACTCATGGCTGCCAAGATAGGC |
| TDR | 20,182,363 | 20,182,385 | AGCAGTGAGGACACGTAAAGGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTCTGAGTAGTAGTAAAGC |
| SD | CATACTCTTGTACCACCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 325 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
