11473 Zfp353 Status: Cancelled
| Gene | Zfp353 |
| Chromosome | 8 |
| Deletion Start | 42,083,106 |
| Deletion End | 42,081,401 |
| Deletion Size | 1,706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,082,666 | 42,082,686 | ACAGCCATGTGTGAGAATCAA |
| TUP | 42,082,618 | 42,082,647 | CATCAAATTGCTCTCTATGGAAGCCATGCA |
| TUR | 42,082,570 | 42,082,593 | CCAGTCATGTTCAGAAGCAAAGAT |
| TDF | 42,081,631 | 42,081,649 | CAGGTGTGATGAGCCTGGA |
| TDP | 42,081,604 | 42,081,630 | TGCACATGGTCGTTTTTCCGCCTATGT |
| TDR | 42,081,566 | 42,081,587 | CTCTCACCACTGTGCTTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGGAGGCAGATATAAGG |
| SD | AATCCAAACTACAATAAGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 280 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
