11474 Mfap3l Status: ES cell colonies screened / QC no positives
| Gene | Mfap3l |
| Chromosome | 8 |
| Deletion Start | 60,656,576 |
| Deletion End | 60,672,016 |
| Deletion Size | 15,441 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,656,636 | 60,656,660 | TGTGCCCTTCTTAATCCTAGTATCC |
| TUP | 60,656,663 | 60,656,688 | TCTAGCCACTGCTAAGAGTGTGACCA |
| TUR | 60,656,707 | 60,656,727 | CAGAGCCCAAGACCACATCAG |
| TDF | 60,671,167 | 60,671,190 | GACATGGGCGTGTACTATATGGTG |
| TDP | 60,671,192 | 60,671,213 | TGTGCCTCGTGGCCTTCACCAT |
| TDR | 60,671,224 | 60,671,243 | CACAGGCGGGTGATGTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CAGATTCTTCTTGAACAAGTAC |
| SD | TCTACAGTTCAGATCACACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 251 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 275 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
