11481 Mfrp Status: Cancelled
| Gene | Mfrp |
| Chromosome | 9 |
| Deletion Start | 44,101,896 |
| Deletion End | 44,106,498 |
| Deletion Size | 4,603 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,102,006 | 44,102,030 | GCCCATAAGACTCTACCTTCCATTC |
| TUP | 44,102,034 | 44,102,063 | ACAGAGTTCTGTAATCCTGCTTTCGACCCG |
| TUR | 44,102,093 | 44,102,112 | TGCCGACATCCCTCTGTAAC |
| TDF | 44,106,077 | 44,106,097 | GCTACAATACCACGGCCTTTC |
| TDP | 44,106,098 | 44,106,120 | CTAACATCTGGGTGGGCCTGGCC |
| TDR | 44,106,133 | 44,106,152 | CCTCGGAGGATGTCTGTCAC |
PCR Primers
| Primer | Sequence |
| SU | TGAATTCCTTCTCGCATCAG |
| SD | CAGAATTGGAACTCTGGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 365 bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
