11482 Prss40 Status: Cancelled
| Gene | Prss40 |
| Chromosome | 1 |
| Deletion Start | 34,560,901 |
| Deletion End | 34,552,470 |
| Deletion Size | 8,432 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,560,216 | 34,560,238 | CCTCTGTCAACTTCAGCGTCTTC |
| TUP | 34,560,188 | 34,560,214 | TCTTCAGAGCTGAGTTCCCTAAGCTGA |
| TUR | 34,560,160 | 34,560,179 | CACGGTGTTGCCAGGTTCTC |
| TDF | 34,553,158 | 34,553,179 | GGGACAAGGGATTCAGCCAAAG |
| TDP | 34,553,133 | 34,553,155 | TACAGGCTGTGCCTCCAGGTCAA |
| TDR | 34,553,107 | 34,553,129 | GTCCCAAAGGGTTGGTAATTGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAATTGTTTCCGTGAAAGC |
| SD | GCTGGAAACAGCTTCTGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 293 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 308 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
