| Gene | Taar4 |
| Chromosome | 10 |
| Deletion Start | 23,960,497 |
| Deletion End | 23,961,787 |
| Deletion Size | 1,291 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,960,719 | 23,960,741 | GCTACCACAGACTTCCTGTTGAG |
| TUP | 23,960,743 | 23,960,767 | TGCGTGGTCATGCCCTTCAGTATGA |
| TUR | 23,960,779 | 23,960,799 | TCCGAAGTACCAGCATGACTC |
| TDF | 23,961,454 | 23,961,475 | CGAAAGGCCCTGAGGATGATAG |
| TDP | 23,961,476 | 23,961,500 | TCACAGGAACGATCTTCCGCTCTGA |
| TDR | 23,961,506 | 23,961,526 | CAGGATGCAGGCTTGAGGTAG |
PCR Primers
| Primer | Sequence |
| SU | GGGAAACGAATTCTGGTCAG |
| SD | GTAATTCAGGCCTCAGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11487A-A1 | VGB6 | C57BL/6NTac | |
| 11487A-H6 | VGB6 | C57BL/6NTac | |
| 11487A-F1 | VGB6 | C57BL/6NTac | |
