11490 Adam19 Status: Germline transmission achieved
| Gene | Adam19 |
| Chromosome | 11 |
| Deletion Start | 45,869,619 |
| Deletion End | 45,878,580 |
| Deletion Size | 8,962 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | ATCCTGGTGGTCAGCTTACTC |
| TUP | | | TGGACCAGACCGACGGCTACC |
| TUR | | | TGCTGTGTTTCCTGGCTTTC |
| TDF | | | GAGTCACGGCAAGCATTCTG |
| TDP | | | TCTCCGCCTCTCAAGTGCTCCA |
| TDR | | | GAGCAAACACAAGCCACCTG |
PCR Primers
| Primer | Sequence |
| SU | CTTCAGAAATTCGGATCATCC |
| SD | GGAAATCCAAGCAGGATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 439 bp | - | + | + |
| NeoFwd | SD | 370 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
