11490 Adam19 Status: Germline transmission achieved
| Gene | Adam19 |
| Chromosome | 11 |
| Deletion Start | 116,941,972 |
| Deletion End | 116,949,771 |
| Deletion Size | 7,800 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,942,013 | 116,942,033 | ATCCTGGTGGTCAGCTTACTC |
| TUP | 116,942,035 | 116,942,055 | TGGACCAGACCGACGGCTACC |
| TUR | 116,942,066 | 116,942,085 | TGCTGTGTTTCCTGGCTTTC |
| TDF | 116,948,841 | 116,948,860 | GAGTCACGGCAAGCATTCTG |
| TDP | 116,948,861 | 116,948,882 | TCTCCGCCTCTCAAGTGCTCCA
|
| TDR | 116,948,905 | 116,948,924 | GAGCAAACACAAGCCACCTG |
PCR Primers
| Primer | Sequence |
| SU | AGCGTGAGGTGTAAGAACAG |
| SD | GAGAGGAACAAGAAGAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 54 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
