11493 Asb12 Status: ES cell colonies screened / QC positives
| Gene | Asb12 |
| Chromosome | X |
| Deletion Start | 95,483,841 |
| Deletion End | 95,482,511 |
| Deletion Size | 1,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,471,314 | 95,471,335 | TCATCTGGAGTGCGTGAGAATG |
| TUP | 95,471,285 | 95,471,311 | TTTAGAAGCTGGTGCCTGTCCTAGTGG |
| TUR | 95,471,244 | 95,471,264 | GTGAGGCAGTGAGAACAGGAG |
| TDF | 95,470,425 | 95,470,446 | TCCCAGACCCGTTTAGTTATCC |
| TDP | 95,470,402 | 95,470,423 | CAGATCCCTCTGCCGGGCGAAC |
| TDR | 95,470,379 | 95,470,397 | AGCTGGTCGGTGGCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | GAAACTGGGAAACAGACACC |
| SD | TGCTCTTCCTTTCCTATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 12143 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 12504 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
