11496 Rnf170 Status: Cancelled
| Gene | Rnf170 |
| Chromosome | 8 |
| Deletion Start | 26,120,487 |
| Deletion End | 26,141,137 |
| Deletion Size | 20,674 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,121,187 | 26,121,206 | TGGGTGCCTCTGGATCTTTG |
| TUP | 26,121,207 | 26,121,233 | TCAGACTAGAGGTCCAGCATTCCATGC |
| TUR | 26,121,311 | 26,121,329 | GGCAAGGCCGAAGTCAATG |
| TDF | 26,140,169 | 26,140,192 | TGAGAGAGGGTCTTATTCTGTAGC |
| TDP | 26,140,200 | 26,140,227 | AGCCTTGAGCTATTACTGATCTCCTGCC |
| TDR | 26,140,252 | 26,140,271 | GTAGCCAGGAGTGGTGGTTC |
PCR Primers
| Primer | Sequence |
| SU | TCATTGAGGTGCATCCTTGC |
| SD | GCACACACCGAGACTCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 604 bp | - | + | + |
| TUF | TUR | 143 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 463 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
