11497 Snai3 Status: ES cell colonies screened / QC no positives
| Gene | Snai3 |
| Chromosome | 8 |
| Deletion Start | 119,906,026 |
| Deletion End | 119,892,410 |
| Deletion Size | 5,793 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,460,011 | 122,460,030 | TCTGGTGGTCTTCGCTCTTC |
| TUP | 122,459,987 | 122,460,010 | CCAGAAGCCTCCTTGCTCTCCTGG |
| TUR | 122,459,965 | 122,459,984 | CGGCTCTTCCTTGGTCACTG |
| TDF | 122,454,979 | 122,454,996 | TTCGCTGACCGCTCCAAC |
| TDP | 122,454,955 | 122,454,977 | TGAGGGCTCACCTGCAGACTCAC |
| TDR | 122,454,927 | 122,454,946 | CGGCGCACCTGTACTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGTGGTGCGCTATGC |
| SD | GGTCATTCCAAGCTGCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 2554859 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 2562224 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
