11506 Nyx Status: Cancelled
| Gene | Nyx |
| Chromosome | X |
| Deletion Start | 13,468,196 |
| Deletion End | 13,487,339 |
| Deletion Size | 19,144 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,468,911 | 13,468,932 | TTGTTGGCTGGCTTCTGTTTAG |
| TUP | 13,468,939 | 13,468,964 | TTCTTATCCAGCCCAGAACCACCTGC |
| TUR | 13,468,968 | 13,468,990 | CACACTATAAGGAGCACCATCAC |
| TDF | 13,486,853 | 13,486,871 | CGCTTTCCAGCCAGGTTTC |
| TDP | 13,486,872 | 13,486,893 | TTCCTGGGCCGCCTCTTCCTTT |
| TDR | 13,486,912 | 13,486,931 | CCACTCCAGTTGGCAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | CCAAATGGATGGATGTGGTG |
| SD | TGCCCATGTGACAAAAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
