11509 Eif3e Status: Cancelled
| Gene | Eif3e |
| Chromosome | 15 |
| Deletion Start | 43,282,689 |
| Deletion End | 43,250,146 |
| Deletion Size | 32,544 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,282,651 | 43,282,669 | TCGCATCGCGCATTTTCTG |
| TUP | 43,282,626 | 43,282,649 | ATCGGCACCTGGTCTTTCCGCTTC |
| TUR | 43,282,574 | 43,282,593 | CGCCGTCCTCCTTCTCAAAG |
| TDF | 43,250,744 | 43,250,765 | CAGGGCTAAGAGCTGTAGAGAC |
| TDP | 43,250,716 | 43,250,741 | AGCATCTGAGAGAGACAGGAAGGCCA |
| TDR | 43,250,681 | 43,250,701 | GTTAAAGCCACGCTGGTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GACAGAACTTCCAAACAAGAG |
| SD | CTAATGTCAAAACCCTGGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 329 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
