11511 Eif3h Status: Cancelled
| Gene | Eif3h |
| Chromosome | 15 |
| Deletion Start | 51,865,454 |
| Deletion End | 51,842,455 |
| Deletion Size | 23,000 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,864,696 | 51,864,716 | TTCCTCCTGAAGGTTGTGGTG |
| TUP | 51,864,664 | 51,864,688 | TCTTTACCCTGAGCACAGGTGAGGC |
| TUR | 51,864,622 | 51,864,643 | GGTGCCAAATGACCTCAAACTC |
| TDF | 51,843,212 | 51,843,235 | CCCTTACCTACTCATTTCTCTGTC |
| TDP | 51,843,174 | 51,843,203 | TTATTTAGCAGGCAATCAGACCTCTGTGCA |
| TDR | 51,843,134 | 51,843,156 | GACCACTCACCCATCAAACATAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGTAGTCAGAGTGACC |
| SD | GCTAGCAAAACTTACCTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
