11514 Paip1 Status: Cancelled
| Gene | Paip1 |
| Chromosome | 13 |
| Deletion Start | 119,429,136 |
| Deletion End | 119,457,081 |
| Deletion Size | 27,946 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,429,595 | 119,429,613 | CGGAGCGCACAAAGGATTC |
| TUP | 119,429,570 | 119,429,593 | AGCCACCACCTCCTTCCAAACCCG |
| TUR | 119,429,549 | 119,429,569 | GGGTAGGGACTAGCCAGGAAC |
| TDF | 119,456,422 | 119,456,443 | GAAGCCCGATGACCTGTGTTTG |
| TDP | 119,456,445 | 119,456,468 | TGTCTGGAACTCCCATGTCGGGTG |
| TDR | 119,456,498 | 119,456,517 | GAGGTGCCTACGTGGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGCGGGACTTAAGGGAGAG |
| SD | ATACACATTTTAAGTTACAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
