11515 1110034A24Rik Status: ES cell colonies screened / QC positives
| Gene | 1110034A24Rik |
| Chromosome | 12 |
| Deletion Start | 70,299,269 |
| Deletion End | 70,290,811 |
| Deletion Size | 8,459 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,299,031 | 70,299,049 | GCCATGTGCTGCGTACCAG |
| TUP | 70,298,997 | 70,299,019 | AGCACCGCTGCTACGTGAACGTG |
| TUR | 70,298,975 | 70,298,994 | CGCCTACCAGTGAGTTGCTG |
| TDF | 70,291,198 | 70,291,215 | CTGTGGACCTGCAACACG |
| TDP | 70,291,170 | 70,291,195 | TGTCCTGAGGTTCCTCACGTACTGTC
|
| TDR | 70,291,124 | 70,291,148 | TATAAACTCAGGATCCATTTTTGCC |
PCR Primers
| Primer | Sequence |
| SU | CAGTGGAGCCTATTTCTAGC |
| SD | CTGACCTAAACTGACTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 330 bp | - | + | + |
| TUF | TUR | 57 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 215 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11515B-A8 | VGB6 | C57BL/6NTac | |
| 11515B-B5 | VGB6 | C57BL/6NTac | |
| 11515B-F4 | VGB6 | C57BL/6NTac | |
| 11515B-G8 | VGB6 | C57BL/6NTac | |
| 11515B-G10 | VGB6 | C57BL/6NTac | |
| 11515B-H12 | VGB6 | C57BL/6NTac | |
