11516 Nr2f1 Status: Cancelled
| Gene | Nr2f1 |
| Chromosome | 13 |
| Deletion Start | 78,198,570 |
| Deletion End | 78,189,683 |
| Deletion Size | 8,888 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,198,289 | 78,198,306 | TCGAGCGGCAAGCACTAC |
| TUP | 78,198,263 | 78,198,285 | CAATTCACCTGCGAGGGCTGCAA |
| TUR | 78,198,209 | 78,198,227 | TTGGCACGGCATGTGTAAG |
| TDF | 78,189,857 | 78,189,874 | TCGCACAGTGTCCTCCTC |
| TDP | 78,189,829 | 78,189,856 | TGTCATCGAGCAACTCTTCTTCGTACGT |
| TDR | 78,189,772 | 78,189,794 | GCTCCCTGACAGCAACATATCTC |
PCR Primers
| Primer | Sequence |
| SU | AGAGAGAAAGAAAACAGCAGG |
| SD | TCTCACCAGACACGAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 402 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
