| Gene | Fth1 |
| Chromosome | 19 |
| Deletion Start | 9,982,856 |
| Deletion End | 9,984,959 |
| Deletion Size | 2,104 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,982,896 | 9,982,914 | GCGCCAGAACTACCACCAG |
| TUP | 9,982,916 | 9,982,936 | ACGCGGAGGCTGCCATCAACC |
| TUR | 9,982,975 | 9,982,994 | GCCGCACTCACCATAGACAG |
| TDF | 9,984,492 | 9,984,512 | TGGCTCAGTGTGAGGTGTTTC |
| TDP | 9,984,518 | 9,984,546 | TTGGGTTGATTGGTAGATGACTTGACCCG |
| TDR | 9,984,557 | 9,984,577 | GTCATCACGGTCTGGTTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTCGCCTGACGCAGGATC |
| SD | TGATTAGTATAACTGGCGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 285 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11518A-H1 | VGB6 | C57BL/6NTac | |
| 11518A-G1 | VGB6 | C57BL/6NTac | |
| 11518A-F2 | VGB6 | C57BL/6NTac | |
| 11518A-A6 | VGB6 | C57BL/6NTac | |
| 11518A-A5 | VGB6 | C57BL/6NTac | |
