11519 Dph2 Status: Germline transmission achieved
| Gene | Dph2 |
| Chromosome | 4 |
| Deletion Start | 117,564,458 |
| Deletion End | 117,562,125 |
| Deletion Size | 2,334 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,564,391 | 117,564,409 | GCGTTCCAGGACAGTTCAC |
| TUP | 117,564,369 | 117,564,390 | TCCTCCTGAAGACCTGGACCGC |
| TUR | 117,564,348 | 117,564,368 | AACTCGCTCCAGCTCATACAC |
| TDF | 117,563,087 | 117,563,105 | GCCTTGATCCCGACCTGAG |
| TDP | 117,563,062 | 117,563,086 | CAGACTGCTCTTGGGTTGGACACCA |
| TDR | 117,563,013 | 117,563,034 | GGTCTTGTGTCTGTCCTGTATC |
PCR Primers
| Primer | Sequence |
| SU | GAGGCTAGTGATTGGTTCTC |
| SD | GGTCTGGATCATCCAACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 326 bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11519A-B2 | VGB6 | C57BL/6NTac | |
| 11519A-B8 | VGB6 | C57BL/6NTac | |
| 11519A-D8 | VGB6 | C57BL/6NTac | |
| 11519A-F7 | VGB6 | C57BL/6NTac | |
