11520 Tmem204 Status: ES cell colonies screened / QC no positives
| Gene | Tmem204 |
| Chromosome | 17 |
| Deletion Start | 25,080,540 |
| Deletion End | 25,058,178 |
| Deletion Size | 22,363 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,080,348 | 25,080,367 | AAGCCCTGGGACTCGAACTG |
| TUP | 25,080,316 | 25,080,341 | TGGACACCCATGACTGTGAGGTTCTA |
| TUR | 25,080,275 | 25,080,293 | CCTCGGGACTCCTGGAAAC |
| TDF | 25,058,477 | 25,058,500 | TCTTAACCTCTCCTCTCCTTCCTG |
| TDP | 25,058,454 | 25,058,476 | CAGGCTTCGTGCTGGTCATCGGA |
| TDR | 25,058,418 | 25,058,439 | GTTGGTGTAAGGGCCAATTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTCAAGAGGCAGGCTAAGAC |
| SD | TCACATGACGTGAAGCCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 332 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
