11522 Bglap-rs1 Status: Cancelled
| Gene | Bglap-rs1 |
| Chromosome | 3 |
| Deletion Start | 88,384,412 |
| Deletion End | 88,383,621 |
| Deletion Size | 792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,384,102 | 88,384,119 | GCCCCCAAATCCCCTTGG |
| TUP | 88,384,075 | 88,384,097 | TGACTGGGTGTCCCCACTTTTCC |
| TUR | 88,384,043 | 88,384,066 | ACACACAAGGTCAGGTAATTCTGG |
| TDF | 88,383,943 | 88,383,964 | CAGAGCCCTTAGCCTTCCATAG |
| TDP | 88,383,917 | 88,383,942 | TGGTAGGAAGGAGTTGTGCTGGAGTG |
| TDR | 88,383,895 | 88,383,914 | GCCCTCTGCAGGTCATAGAG |
PCR Primers
| Primer | Sequence |
| SU | ATTGTGGCCTCTCGTCCAC |
| SD | TTGCTATCCATCACAAAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
