11525 Litaf Status: ES cell colonies screened / QC no positives
| Gene | Litaf |
| Chromosome | 16 |
| Deletion Start | 10,966,641 |
| Deletion End | 10,960,923 |
| Deletion Size | 5,719 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,966,420 | 10,966,439 | CCAACGCCAACGCAAGTATG |
| TUP | 10,966,394 | 10,966,419 | TCTGTTATCCAGGTTCTTGCAGCTCC |
| TUR | 10,966,362 | 10,966,380 | TCCCTCCTGGAGTCCACAC |
| TDF | 10,961,293 | 10,961,312 | CTAAGGCTGGCCTCCAACTG |
| TDP | 10,961,262 | 10,961,291 | CCCAGGTAGTCAGAAATGACCTTGAACTCC |
| TDR | 10,961,238 | 10,961,260 | GCAGGAATACAGGCAGGAGAATC |
PCR Primers
| Primer | Sequence |
| SU | GTGATAATTTCCTTGACTGTTC |
| SD | AACATGAGCAGGCAGGAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 419 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
