Gene | Smad5 |
Chromosome | 13 |
Deletion Start | 56,723,597 |
Deletion End | 56,737,536 |
Deletion Size | 13,940 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 56,724,152 | 56,724,175 | TGCCTGCTATTGATAAGCACTTTC |
TUP | 56,724,182 | 56,724,207 | AACACCCATTTCCTTTCTGCGGCCTA |
TUR | 56,724,233 | 56,724,255 | AGGACCTCGTGTGAATATGGAAC |
TDF | 56,737,014 | 56,737,036 | ATGAGGGCGTGGGCTTTATTTAC |
TDP | 56,737,037 | 56,737,065 | TCAGTGAGAATATGTATCACAGCACGGCA |
TDR | 56,737,073 | 56,737,094 | CCAGACTATGATCCTTCACAGC |
PCR Primers
Primer | Sequence |
SU | TTAGACTGAATCAGGCTCCC |
SD | AAGTACGTTCCAGTTTAAGATC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 335 bp | - | + | + |
TUF | TUR | 104 bp | + | + | - |
TDF | TDR | 81 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
11528A-H3 | VGB6 | C57BL/6NTac | |
11528A-H4 | VGB6 | C57BL/6NTac | |