11529 Cyp19a1 Status: Cancelled
| Gene | Cyp19a1 |
| Chromosome | 9 |
| Deletion Start | 54,193,398 |
| Deletion End | 54,166,753 |
| Deletion Size | 26,646 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,192,737 | 54,192,756 | GCAGCGTGGTGATGGTGATG |
| TUP | 54,192,709 | 54,192,735 | TGCCATAAGGGTTGAATGAGAACTGGC |
| TUR | 54,192,680 | 54,192,700 | TGGGCCTTGTCCCTATTCTTC |
| TDF | 54,167,167 | 54,167,185 | TGGCCGGTCTGTGTCATAG |
| TDP | 54,167,139 | 54,167,165 | AGAGGCACAGTAGCTTCGTTAGCCACA |
| TDR | 54,167,101 | 54,167,120 | GCATTCAGCCATGCTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | TGTTGAACAGAACCTGAGCC |
| SD | TAGGATTGGTCCTTTAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
