11532 Mrps33 Status: Cancelled
| Gene | Mrps33 |
| Chromosome | 6 |
| Deletion Start | 39,805,582 |
| Deletion End | 39,802,420 |
| Deletion Size | 3,163 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,804,971 | 39,804,992 | TTGCCTCTTACTGGGTGTGTAG |
| TUP | 39,804,947 | 39,804,969 | AGCCTCCGTTCTCTGCTGGTGGC |
| TUR | 39,804,913 | 39,804,935 | GCGAGTGGGAGGAATAAGAAATG |
| TDF | 39,803,006 | 39,803,028 | GAACTGCTTTCCATGTGCTTGAC |
| TDP | 39,802,976 | 39,803,001 | TTCCCATTCATGCTCTTCCGGACAAC |
| TDR | 39,802,941 | 39,802,961 | GACTGCCGAGGAATTAGCATC |
PCR Primers
| Primer | Sequence |
| SU | AAGAGACAGTCCGTGAACAC |
| SD | CTCCATTATAGAATTAGGTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 348 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 310 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
