11533 Pabpc5 Status: ES cell colonies screened / QC no positives
| Gene | Pabpc5 |
| Chromosome | X |
| Deletion Start | 119,927,993 |
| Deletion End | 119,929,153 |
| Deletion Size | 1,161 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,928,069 | 119,928,088 | ACGTCACAGAGGACATGCTC |
| TUP | 119,928,091 | 119,928,116 | TAAGAAGTTCAGACCTGCTGGCCCTC |
| TUR | 119,928,129 | 119,928,148 | CACTGGGTCACGACAGATTC |
| TDF | 119,928,653 | 119,928,674 | GGGCCAACTGAGAGTGTTAAAG |
| TDP | 119,928,679 | 119,928,706 | AAGAGATGCCACTGGGAAATCTAAAGGC |
| TDR | 119,928,725 | 119,928,745 | TTGGGCAGCTTCATGTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | GGTTGCCTTTTTGTCTGATG |
| SD | TTTCCTCATTCAGGATACTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 377 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
