11535 Ppp1r17 Status: ES cell colonies screened / QC no positives
| Gene | Ppp1r17 |
| Chromosome | 6 |
| Deletion Start | 56,022,387 |
| Deletion End | 56,031,566 |
| Deletion Size | 9,180 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,022,423 | 56,022,444 | CTGGAGCTTTCAGACGACATAC |
| TUP | 56,022,445 | 56,022,468 | TAGGCAAGCTGGATCCTCAGTGCA |
| TUR | 56,022,478 | 56,022,498 | TGTGCCAGAGGGAGCATTTAC |
| TDF | 56,030,987 | 56,031,009 | GGAGATCCAACTCAGGCTATCAG |
| TDP | 56,031,010 | 56,031,038 | TGGTAATGACAAGCCACTTTACTGAGCCA |
| TDR | 56,031,040 | 56,031,059 | CACATCTGTGGGCCAGTGAC |
PCR Primers
| Primer | Sequence |
| SU | ATACATGGAAAATAGGACTGAG |
| SD | TCACCTCTCTTACTTTGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 339 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 361 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
