11538 Pax9 Status: ES cell colonies screened / QC no positives
| Gene | Pax9 |
| Chromosome | 12 |
| Deletion Start | 56,695,845 |
| Deletion End | 56,709,910 |
| Deletion Size | 14,066 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,696,008 | 56,696,028 | AGTGGCGGAAAGGGTTCTGTG |
| TUP | 56,696,031 | 56,696,055 | AGCCACTGGGTGACAATTGATGCCA |
| TUR | 56,696,092 | 56,696,111 | ACACTGGAGCTGCGAGATTC |
| TDF | 56,709,644 | 56,709,662 | TCCTCTCAGGCACCGAATG |
| TDP | 56,709,665 | 56,709,688 | CTCCCAGCTGTGAGCAGTTTCGTC |
| TDR | 56,709,708 | 56,709,727 | TTGGGCTGGTGTAGGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | CACAAGTGAGTGATAGACTG |
| SD | AGTTATGCCAGGTTTCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 279 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
