11542 Paf1 Status: Cancelled
| Gene | Paf1 |
| Chromosome | 7 |
| Deletion Start | 28,393,194 |
| Deletion End | 28,399,251 |
| Deletion Size | 6,058 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,393,928 | 28,393,949 | AGCTCTGAGTGGCAGTCTAAAG |
| TUP | 28,393,954 | 28,393,976 | TTCCTTAGGCTGGACAGCAGGCA |
| TUR | 28,393,996 | 28,394,016 | ACCCTGCCTCAAGCTGACAAG |
| TDF | 28,398,839 | 28,398,861 | GAAGGGTAGCAGCAGTGAGAAAG |
| TDP | 28,398,863 | 28,398,888 | AGGCAGCGAGGATGAACACTCTGGAA |
| TDR | 28,398,925 | 28,398,945 | CGCTGCCACTCTTGTCACTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGCGACCTGTCTAGTCTTC |
| SD | GGGAATCCATGGGGACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
