11543 Tmc4 Status: Cancelled
| Gene | Tmc4 |
| Chromosome | 7 |
| Deletion Start | 3,677,369 |
| Deletion End | 3,665,832 |
| Deletion Size | 11,556 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,676,825 | 3,676,844 | GCCTGGACTGTGGATCTGAG |
| TUP | 3,676,798 | 3,676,821 | AAAGGCTTGTGGCCTGGACTCCTC |
| TUR | 3,676,770 | 3,676,794 | TGGGATGAACACAAAGGATTGAAAG |
| TDF | 3,666,176 | 3,666,198 | CATTCCTGTCTTCCATAGCATCC |
| TDP | 3,666,144 | 3,666,172 | TGATGTATACCGTAGCCCTGGCTAACTGT |
| TDR | 3,666,094 | 3,666,113 | TGGCTCACCGTCTCTATCTG |
PCR Primers
| Primer | Sequence |
| SU | TCTGGCTGCCATGAAGTTTG |
| SD | TAAGCAGACATAACCTAGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 435 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 237 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
