11544 Phpt1 Status: ES cell colonies screened / QC positives
| Gene | Phpt1 |
| Chromosome | 2 |
| Deletion Start | 25,430,356 |
| Deletion End | 25,429,081 |
| Deletion Size | 1,276 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,430,294 | 25,430,316 | CGGATGGCGTCTTCAAGTATGTG |
| TUP | 25,430,265 | 25,430,292 | TGATTCGAGTCCACTTAGCAGAGCCTTC |
| TUR | 25,430,229 | 25,430,248 | CGCACGATTTCCTTGCACTC |
| TDF | 25,429,225 | 25,429,245 | CAAACTGGCGGTCATCCTTTC |
| TDP | 25,429,203 | 25,429,224 | TCTAGGGTTACGGTCGTGCCCA
|
| TDR | 25,429,141 | 25,429,160 | CAGCCCAGGTGACCTCATAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTTAACTGGGACTCATGAG |
| SD | CTGAAGCTGAGCAGGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
