11546 S100a10 Status: ES cell colonies screened / QC no positives
| Gene | S100a10 |
| Chromosome | 3 |
| Deletion Start | 93,560,967 |
| Deletion End | 93,564,406 |
| Deletion Size | 3,440 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,561,673 | 93,561,694 | TCTTAGGAGCCTGGAAGCACAG |
| TUP | 93,561,705 | 93,561,731 | AGCCCACTTTGGGAAGATTGAACAGGA |
| TUR | 93,561,774 | 93,561,796 | GGCCTAGAAAGTCAAAGGACAAC |
| TDF | 93,564,310 | 93,564,331 | TGGGCTTCCAGAGCTTTCTATC |
| TDP | 93,564,333 | 93,564,356 | CTAGTGGCAGGGCTCACCATTGCA |
| TDR | 93,564,375 | 93,564,396 | TCCCCTTCTGCTTCATGTTTAC |
PCR Primers
| Primer | Sequence |
| SU | GCATATAGAGTGGCTGACAG |
| SD | CACTCTACGTCAAATTCAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 375 bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 354 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
