11548 Nr2f6 Status: ES cell colonies screened / QC no positives
| Gene | Nr2f6 |
| Chromosome | 8 |
| Deletion Start | 71,381,268 |
| Deletion End | 71,375,779 |
| Deletion Size | 5,490 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,380,360 | 71,380,378 | CCGGGTTGGTTCAGCTTTG |
| TUP | 71,380,329 | 71,380,356 | CTCCGGGATGAGACCTTAAACTTCGGGA |
| TUR | 71,380,298 | 71,380,316 | TCTCCCACGGGTACTTCAC |
| TDF | 71,376,619 | 71,376,641 | GCCTTGAGCTTCTGACCTTCTTG |
| TDP | 71,376,593 | 71,376,616 | TCCATGCTCCAGAGCTGGAATGGC |
| TDR | 71,376,557 | 71,376,579 | TGCCATTGTTAGCTTTGCCTATG |
PCR Primers
| Primer | Sequence |
| SU | CAGACTCGCGCCTCTAGC |
| SD | GATGACCTGGTTGCCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
