| Gene | Btbd3 |
| Chromosome | 2 |
| Deletion Start | 138,279,094 |
| Deletion End | 138,284,540 |
| Deletion Size | 5,447 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,279,822 | 138,279,844 | CGGTTGCCAGGACACAAAGTAAG |
| TUP | 138,279,845 | 138,279,873 | CAACAGCTGCATTACCGGTTTAGTCCTGA |
| TUR | 138,279,901 | 138,279,918 | TGCCCACACCAAGCTCTG |
| TDF | 138,283,692 | 138,283,714 | GTCTGAGGGATTCTGTGACATCG |
| TDP | 138,283,719 | 138,283,744 | CCAGACACTCGAAAGTATCCTCCGCA |
| TDR | 138,283,789 | 138,283,811 | CATTCTACCTCAGCCCAGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | GGTCTCTGTTAACATCTTAGC |
| SD | TACAGACGCAACTCGAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 413 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11559A-B12 | VGB6 | C57BL/6NTac | |
| 11559A-B6 | VGB6 | C57BL/6NTac | |
| 11559A-C1 | VGB6 | C57BL/6NTac | |
