| Gene | Mbd6 |
| Chromosome | 10 |
| Deletion Start | 127,287,511 |
| Deletion End | 127,282,828 |
| Deletion Size | 4,684 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,286,854 | 127,286,871 | GCAACCACAGGCGGAAAG |
| TUP | 127,286,823 | 127,286,847 | CCATGGCAACTCTGTACCGCAGCAT |
| TUR | 127,286,795 | 127,286,816 | AGGAGAAGAGTGTGAGCAGGTG |
| TDF | 127,283,131 | 127,283,153 | ACCAGCCTTCTGAAATTCACCTG |
| TDP | 127,283,110 | 127,283,129 | TGCTCGGGAGCTGCTCCTGA |
| TDR | 127,283,085 | 127,283,105 | AGGGCTGCAATAAGGGCACAG |
PCR Primers
| Primer | Sequence |
| SU | TGAGTTGGTAGACTGAGAGG |
| SD | GTCCCCTTAGCGCCTGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 358 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11562A-C8 | VGB6 | C57BL/6NTac | |
| 11562A-D11 | VGB6 | C57BL/6NTac | |
| 11562A-H4 | VGB6 | C57BL/6NTac | |
