11563 Kptn Status: ES cell colonies screened / QC no positives
| Gene | Kptn |
| Chromosome | 7 |
| Deletion Start | 16,119,944 |
| Deletion End | 16,127,245 |
| Deletion Size | 7,302 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,120,115 | 16,120,135 | CGCTATCAAGACCTCCGACAG |
| TUP | 16,120,138 | 16,120,163 | AATCCGGCCTGTGGCTAAAGAGCTGC |
| TUR | 16,120,168 | 16,120,186 | CGCACCGGGAATGTAGTTG |
| TDF | 16,126,514 | 16,126,533 | AGTGGAGCATCTCTGAGGTC |
| TDP | 16,126,538 | 16,126,563 | CTCAGCTTCTGCTTTCCACTTCTGCC |
| TDR | 16,126,573 | 16,126,597 | TGCAACAAATGAGAGGTTAGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCCATCTCAAATCCTGGAAG |
| SD | AGCTTTGTTGCTCTGAATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
