11564 Tmem222 Status: Cancelled
| Gene | Tmem222 |
| Chromosome | 4 |
| Deletion Start | 133,277,721 |
| Deletion End | 133,266,807 |
| Deletion Size | 10,915 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,277,152 | 133,277,170 | GCAAACGAGCGCGTACTTG |
| TUP | 133,277,124 | 133,277,150 | CGTCTGTTATGCACAAGACGTTTCGCC |
| TUR | 133,277,082 | 133,277,104 | AACCAGTGTGGCTTCGATCAAAC |
| TDF | 133,266,906 | 133,266,925 | TCAGTGTTGGAGCCTTTGTG |
| TDP | 133,266,881 | 133,266,905 | AAGACCTGGCTGCCCTTTGTCCTTC |
| TDR | 133,266,837 | 133,266,856 | CCGCAGATTGAAGACGAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTTGGCACCTAAGAAGTGATC |
| SD | GAGTAGCCCAGTGATGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
