11565 Amacr Status: Cancelled
| Gene | Amacr |
| Chromosome | 15 |
| Deletion Start | 10,981,795 |
| Deletion End | 10,995,261 |
| Deletion Size | 13,467 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,982,452 | 10,982,471 | CATGCACCTCCATCCGACTG |
| TUP | 10,982,477 | 10,982,504 | TGGTATTTGCAACTGTCACAAACCCTGA |
| TUR | 10,982,540 | 10,982,560 | GGCTGGTTGGAATGGATTCTC |
| TDF | 10,995,082 | 10,995,101 | GCACCTCTGCTTTCCAGAAC |
| TDP | 10,995,103 | 10,995,124 | CCTGCCGTCCCATCTGCCAAAA |
| TDR | 10,995,136 | 10,995,155 | TCTACGGTGTGCTCCCCTAC |
PCR Primers
| Primer | Sequence |
| SU | GATCTAAGGAGTTGAGTGCC |
| SD | AATGGATACACTTCACCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 408 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
