11566   Gbas     Status: ES cell colonies screened / QC no positives

GeneGbas
Chromosome5
Deletion Start129,725,130
Deletion End129,757,151
Deletion Size32,023
Genome Build38
MGI IDMGI:1278343
NCBI Gene ID14467
BAC ID398P21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF129,725,472129,725,490GCCCCTCGCAGTAGCAATG
TUP129,725,492129,725,512CTCGCCCCAGGTCGTGCAGAG
TUR129,725,516129,725,536GGGAGACCAGCCATCCTTTTG
TDF129,756,236129,756,256AGTGCAGGTAGGAGGCTCCAG
TDP129,756,258129,756,283TCTGCGCGGTCCTTGGTTACATAGAA
TDR129,756,304129,756,323TAGCCGCACTGTTGTGTTTC

PCR Primers

PrimerSequence
SUACTTCAGAGACCCTGAACTC
SDATGATCCTGTTTTCCTGAAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev361 bp-++
TUFTUR65 bp++-
TDFTDR88 bp++-
NeoFwdSD312 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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