11568 Ccdc92 Status: Cancelled
| Gene | Ccdc92 |
| Chromosome | 5 |
| Deletion Start | 124,839,787 |
| Deletion End | 124,835,419 |
| Deletion Size | 4,369 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,839,146 | 124,839,167 | TCTCCCAGCCTTGATATGAACC |
| TUP | 124,839,112 | 124,839,139 | CCTGCCTGGTGTTTCTTACATTGATCCC |
| TUR | 124,839,070 | 124,839,094 | GTGAGTGAGTTTGACCCTGTAGAAG |
| TDF | 124,836,320 | 124,836,340 | TCTCCTCCCTCAGCTAGACTC |
| TDP | 124,836,299 | 124,836,319 | TGACCGGGCGCCTGACTTGTT |
| TDR | 124,836,245 | 124,836,265 | CTCCTCACAGCGCCTCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TGCTTCCTCTCCAGAACAAG |
| SD | GATAGCTCCTTCTCTGCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 395 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
