11569 Cd72 Status: ES cell colonies screened / QC no positives
| Gene | Cd72 |
| Chromosome | 4 |
| Deletion Start | 43,454,524 |
| Deletion End | 43,448,237 |
| Deletion Size | 6,288 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,454,298 | 43,454,319 | CCTACGAGAATGTGCAAGTGTC |
| TUP | 43,454,278 | 43,454,297 | TCCAGTCCCAGGAGGGCCAC |
| TUR | 43,454,234 | 43,454,252 | TGCCCTACCTGCTTTGTCC |
| TDF | 43,448,800 | 43,448,819 | CCAGAGGAGTGCTGGCATTG |
| TDP | 43,448,774 | 43,448,798 | CCTCACTAAGCCTTGACAGGCTGCC |
| TDR | 43,448,753 | 43,448,773 | GGCGTAGACTGAGCAAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTTAAGTGGGTCTAGGATG |
| SD | GAGTGCTGGAGGAATAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 445 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
