11575 Elovl3 Status: ES cell colonies screened / QC one positive
| Gene | Elovl3 |
| Chromosome | 19 |
| Deletion Start | 46,132,053 |
| Deletion End | 46,134,810 |
| Deletion Size | 2,758 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,132,168 | 46,132,189 | GTACTGGTGAGACCTGGTGATG |
| TUP | 46,132,193 | 46,132,220 | AAGGACCAACTGCTTTGAGGGAAATGGA |
| TUR | 46,132,237 | 46,132,257 | GCGTCGTCGGAGACTTTACAG |
| TDF | 46,134,340 | 46,134,358 | GGGTGGCCTGACGACTATG |
| TDP | 46,134,363 | 46,134,386 | CTCACCTCTCAGGAGACACGGCCT |
| TDR | 46,134,391 | 46,134,409 | TGGACGCTTACGCAGGATG |
PCR Primers
| Primer | Sequence |
| SU | ATTGGATAACTGCTGAGGGC |
| SD | ACCTCAGTCCTATCTCCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 314 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
