11578 Pole Status: Cancelled
| Gene | Pole |
| Chromosome | 5 |
| Deletion Start | 110,310,610 |
| Deletion End | 110,337,242 |
| Deletion Size | 26,650 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,287,187 | 110,287,206 | GTGAAGCTCTGGGTTTGCTG |
| TUP | 110,287,208 | 110,287,231 | ACAACACCTGCATAGCAGCCAGGG |
| TUR | 110,287,248 | 110,287,268 | TGTCACTGAAGAACCGCTTAC |
| TDF | 110,312,545 | 110,312,567 | AGCCCTTAGGCTGCTCTTTGATG |
| TDP | 110,312,572 | 110,312,601 | AAAGCTGGGTACTACCTTAACCATCCACTG |
| TDR | 110,312,670 | 110,312,694 | GTTACAGGCCAAGATTACTAAATGC |
PCR Primers
| Primer | Sequence |
| SU | CACCCAACAGCTAGGCAGTC |
| SD | TGCAAATGAGTTCTTCATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 24703 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | 24036 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
