11579   Pabpc2     Status: ES cell colonies screened / QC no positives

GenePabpc2
Chromosome18
Deletion Start39,773,670
Deletion End39,775,592
Deletion Size1,923
Genome Build38
MGI IDMGI:1349723
NCBI Gene ID18459
BAC ID439O22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 18

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF39,773,82739,773,846ACGCCGTTCCCTGGGCTATG
TUP39,773,84739,773,871CTTCCGTGAACTTTGAGCAGCCTGC
TUR39,773,91139,773,929GACCGGCTTGCCCTTGATG
TDF39,775,01739,775,036CCAATGTGCCTGGTGCTATC
TDP39,775,03739,775,060CACCCAGCTGCTCCTAGATCGTCG
TDR39,775,08639,775,105TGTCGTGACTTGAACGTGTG

PCR Primers

PrimerSequence
SUAGCAGACAGTCCTTGCTCTC
SDACATTGGTTTGTAGATCCTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev370 bp-++
TUFTUR103 bp++-
TDFTDR89 bp++-
NeoFwdSD292 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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